The Kota Johor Lama Museum (Malay: Muzium Kota Johor Lama) is a museum in Johor Lama, Kota Tinggi District, Johor, Malaysia. The museum is about the history of Johor Lama.
The museum started to be constructed in August 2004 and completed in February 2007. It was opened to the public in October 2007.
The museum resembles a traditional house.